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The reaction outcome is determined by measuring the ratio of fluorescence emission at 445 nm (coumarin) and 520 nm (fluorescein), corresponding to the relative amounts of cleaved and intact peptides

J Biol Chem 1998;273:1747782

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Inhibition of 5-HT3 receptor-mediated ion current by divalent metal- cations in NCB-20 neuroblastoma-cells