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Kinetic fluorescence measurements were performed in a microplate reader at an excitation wavelength (ex) of 389 nm and an emission wavelength (em) of 513 nm, with a runtime of 15 min at 1-min intervals and a temperature set to 37 C to determine the average velocity (Vmean) of the enzymatic reaction

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Such information is crucial for developing novel treatments aimed at mitigating the inflammatory response and reducing liver damage in patients with APAP-induced liver injury