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Species verification PCR amplification was performed using ITS2 forward primer (5-TGTGTCGATGAAGAGCGCAG) and ITS2 reverse primer (5-TGGTTAGTTTCTTTTCCTCCGC), as described previously (Morphew et al ., Reference Morphew, Eccleston, Wilkinson, McGarry, Perally, Prescott, Ward, Williams, Paterson, Raman and Ravikumar2012)

We took human hepatic cell line LO2 and hepatocellular carcinoma cell line HepG2 to establish the models of steatosis hepatocyte induced by alcohol, taken PGPIPN as pharmacological intervention

Keystone, E., Wherry, J

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This medication is suitable for patients who still have some natural insulin production ability in their pancreas

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