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Lysates were centrifuged at 13000 g , 15 min, and 4 C to separate and remove cell debris
FAQs Q: What is whey protein and how does it benefit muscle growth
The YAK1 -KD (kinase dead, p.K398R) and MCK1 -KD (p.K68R) mutants were created by site-directed mutagenesis (QuickChange Lightning, Agilent technologies, Santa Clara, CA, USA) according to the manufacturer instructions using primers listed in Supplementary Table 2
Gene expression of p63 , which encodes transformation-related protein 63 (p63), was measured because it is essential for the proliferative capacity of the stem cells 11 , while the expression of Axin2 , which is a Wnt-target gene 10 , was measured to assess Wnt signalling activity in the 3D culture
Helps reduce oxidative stress, supporting healthy ageing
Harding JL, Sooriyakumaran M, Anstey KJ, Adams R, Balkau B, Brennan-Olsen S, Briffa T, Davis TM, Davis WA, Dobson A, et al
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