Ischemia and reperfusion injury are powerful generators of ROS and OS
5.2 Metabolite intervention 5.2.1 N-acetylcysteine N-Acetylcysteine (NAC), as a classic antioxidant, has shown potential as a therapeutic option for systemic autoimmune diseases in recent years
Methods Rats were randomly divided into the control, morphine and BPC-157 groups
Western blotting Cell pellets were lysed in RIPA buffer (25 mM Tris-HCl, pH = 7.5, 150 mM NaCl, 0.1% Nonidet P-40, 0.5% sodium deoxycholate and 0.1% SDS) supplemented with protease inhibitor cocktail (Sigma) and phosphatase Inhibitor Cocktail 2 and 3 (Sigma)
10.1002/med.21419 248 RanadeS
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