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2.15.2 Neural function defect score (NSS) Twenty-four hours post-infarction, neurological deficits were scored using the Masao Shimizu Sasamata method ( 2.16 Immunohistochemical analysis Mice were humanely sacrificed by deep anesthesia using an overdose of pentobarbital, followed by transcardial perfusion with phosphate-buffered saline (PBS) to clear the blood

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doi: 10.1002/adhm.202100149 72 IngoldIBerndtCSchmittSDollSPoschmannGBudayKet al

In addition, Owen and colleagues and Cho and colleagues observed that ECOG performance status was not associated with immune-related adverse events