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More serious complications are rare but possible

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The GST tag can easily be removed following purification by addition of a protease if a suitable protease-cleavage site has been inserted between the GST-tag and the protein of interest (which is usually included in many commercially available sources of GST-tagged plasmids)

The process of titration is like climbing a ladder: you start at the bottom (low dose) and take one step at a time (gradual dose increase) until you reach your goal (optimal blood glucose control with minimal side effects)

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