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Subsequently, cells were incubated with secondary antibodies: PE-conjugated goat anti-mouse IgG (H + L) (for -tubulin, 1:500) and FITC-conjugated goat anti-rabbit IgG (H + L) (for NRF2, 1:500) for 1 h at RT in the dark

Specifically, we sought to determine the impact of estrous cycle phase (proestrus, estrus, metestrus and diestrus) on the response to a single dose of semaglutide (0.01 mg/kg) over a 24-h period of observation

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This is not fully understood, and it should be discussed with a medical professional rather than assumed for any individual