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By Day 45 of iHLOs, proteomic data signaled a transition toward mesenchymal differentiation

These enzymes protect cells from oxidative damage, a major contributor to aging and disease

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Vegans and vegetarians: Since B12 is primarily found in animal products, supplementation is often necessary

Secondary outcomes: ADL-functioning, neuropsychological tests and measures of quality of life

The BIOM file output from the DADA2 pipeline and the DECIPHER-aligned FastTree phylogenetic tree were input into QIIME (v1.9.1) and the scripts in the Supplementary Materials (QIIME Scripts compilation) were used to establish the alpha diversity metrics of Chao1 richness estimate, Simpsons evenness measure E, Shannon diversity index H, Simpsons diversity index, and Faiths phylogenetic diversity metric (whole tree) as well as the beta diversity measures of unweighted and weighted UniFrac analyses (the latter of which factors in ASV abundance 37 ) and BrayCurtis dissimilarity analysis