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A thicker needle (a lower gauge number) creates more trauma at the injection site, which can be a confounding variable in itself, especially in studies looking at healing or inflammation

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Plasmid construction and generation of transgenic and gene silencing plants To screen SsVSPs, the full-length coding region (SsVSPs FL ) or the sequence with the predicted SP removed and artificial start codon added at the N-terminal (SsVSPs SP ) was cloned into the pCHF1301-3FLAG vector

Any additional information required to reanalyze the data reported in this paper is available from the corresponding author upon request

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By analysing small-molecule metabolites in biological systems, metabolomics can offer a window into the biochemical changes underlying epilepsy