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Progress in Lipid Research , 47(4), 318-336

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The process is easy, safe, and quick

Cystine uptake assay Cells were washed three times with pre-warmed sodium-free uptake buffer (137 mM choline chloride, 3 mM KCl, 1 mM CaCl 2 , 1 mM MgCl 2 , 5 mM d-glucose, 0.7 mM K 2 HPO 4 and 10 mM HEPES, pH 7.4), then incubated with 0.5 ml of pre-warmed uptake buffer at 37 C for 10 min