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The spheres were washed twice and stained with 1:500 PI solution (Bio-Sciences) supplemented with 250 g/mL RNAse A (Qiagen) and Hoechst nuclear counterstain (2 g/mL) (Bio-Sciences) for 30 min protected from light, at room temperature

When to Seek Immediate Care Contact your provider or go to the emergency room if you experience: Severe abdominal pain or a rigid, board-like abdomen High fever (above 101F / 38.3C) with abdominal pain Significant rectal bleeding or blood in stool Signs of bowel obstruction: severe cramping, vomiting, inability to pass gas or stool Sudden worsening of symptoms in a patient on biologic therapy (may indicate infection) About this Guide This Clinical Documentation Guide is published by CCO Academy and is intended for credentialed coding, CDI, and clinical documentation professionals

The above tests can help you get a correct diagnosis

doi:10.1007/978-0-387-78518-9_18