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If you live in a climate-controlled home at 22C and the vial sat out for four hours, you've lost roughly 1015% potency

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Cell culture and STR analysis ARPE-19 human RPE cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA), and cultured in DMEM/F12 medium supplemented with 10% fetal bovine serum at 37 C in air containing 5% CO 2