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Introduction Mitochondria are organelles produced by archaea that are required for the production of ATP ( Studies discovered mitochondrial-derived peptide called MOTS-c has been shown to significantly reduce the level of pro-inflammatory factors in mice and increase anti-inflammatory factors and insulin-stimulated glucose treatment rates, as well as glucose homeostasis ( Physiological function of MOTS-c MOTS-c, one of the newly discovered sORF-encoded peptides, is a 16-amino acid polypeptide encoded by the mitochondrial 12S rRNA gene and localized to mitochondria under resting conditions ( Figure 1 ) ( Figure 1 MOTS-c, as a mitochondrial coding regulator, has endocrine-like and nuclear transcriptional regulation on muscle metabolism ( Originally, MOTS-c was identified in the process of genetic and pharmacological screening of metabolic regulation in human cells ( MOTS-c has also been found to be involved in the regulation of nuclear gene expression by binding to transcription factors ( Effects of MOTS-c against diseases MOTS-c and aging Gradually disordered metabolic level is one of the signs of aging, which inhibit the normal physiological function of the body and even lose the ability to take care of themselves ( Studies have shown that the interaction of MOTS-c/NRF2 can improve the expression of mitochondrial protective genes ( MOTS-c and cardiovascular disease Obesity is the main culprit of cardiovascular problems ( Recent studies have shown a protective effect of MOTS-c against cardiac dysfunction and pathological remodeling (33)

The mice were placed in running wheel cages with ample time to adjust and get trained in using the wheel