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DNA constructs and mutagenesis PCR-amplified human SLC2A1 , DHHC9 , and GCP16 were subcloned into baculoviral vector pFastBac1, lentiviral vector pCDH (hygromycin resistance), bicistronic expression vector pIRES, or green fluorescent protein vector pEGFP-C1

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Next, we measured total iron (Fe) content alongside Copper (Cu), Manganese (Mn) and Zinc (Zn) levels in OC and OTC brain areas of AD patients and control individuals