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By activating both receptors at once, Tirzepatide appears to engage complementary pathways involved in metabolic homeostasis, making it a promising peptide for studies focused on energy regulation

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The separation was carried out on a Welch Ultisil Amino Acid (4.6250 mm, 5 m) column under a gradient program using sodium acetate trihydrate solution: acetonitrile (93:7) as mobile phase A and acetonitrile: water (80:20) as mobile phase B

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