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Compared to somatic cells, sperm cells have fewer resources to neutralize ROS, leaving them more exposed to oxidative damage

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Key pathways, including Wnt/-catenin, bone morphogenetic protein 2/Smad, phosphoinositide 3-kinase/Akt and protein kinase A, play a role in this

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However, questions remain regarding how Astragaloside specifically regulates osteoblast and osteoclast differentiation, which signaling pathways and gene expression changes are involved, its potential adverse effects on other tissues or organs, and dose-dependent toxicity