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Major PK Uncertainties: Translational Scaling : The PK parameters (especially clearance rates and effective doses) observed in small animals do not reliably scale up to humans
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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]