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After repeated washing the bound fraction was eluted with 250 mM imidazole (Fischer) in lysis buffer containing 10% glycerol (Acros)

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Researchers comment that a single introduction of the peptide may upregulate growth hormone production by somatotrophs for prolonged periods of time, thus apparently contributing to an overall increase in [growth hormone] secretion by 46% and also potentially upregulating its main anabolic mediator insulin-like growth factor-1 (IGF-1) by 45% on average

Vitamin C helps convert it back to its reduced, active form (GSH), creating a continuous cycle of protection