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Blood was drawn into BD Vacutainer EDTA tubes (BD Biosciences), and plasma (0.25 ml) was recovered from each sample and stored in a low-protein-binding tube prior to proteomic research

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Sharif A, Hecking M, de Vries APJ, Porrini E, Hornum M, Rasoul-Rockenschaub S, et al

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To test whether the apoptosis caused by suppression of Hh signalling is due to the reduction of Dpp signalling, we examined the dpp expression using a dpp-lacZ reporter

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