Global burden of digestive diseases: A systematic analysis of the global burden of diseases study, 1990 to 2019
3 | Sulfhydryl groups (-SH) measurement Determination -SH wasevaluated according to Ellman'smethod.21 The expe riment is based o n the absorban ce differenc e at 412 nm after photometric reaction between SH and 5,5-dithiobis-(2nitrobenzoic acid)(DTNB).Briefly,colonsupernatantsweremixedwith100Lof SDS(10%)and800Lofphosphatebuffer(10mmol/L,pH8)given the first optical density measurement
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Long-term oral studies of acetylcysteine alone in rats (12 months of treatment followed by 6 months of observation) at doses up to 1,000 mg/kg/day (5.2 times the human mucolytic dose) provided no evidence of oncogenic activity
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