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( A ) cAMP responses in islets isolated from chow vs high cholesterol (high chol) diet fed mice transduced with Green Up cADDis biosensor and stimulated with 100 nM exendin-4 (Ex-4) in 6 mM glucose (G6) media followed by 100 M isobutyl methylxanthine (IBMX) + 10 M Forskolin (FSK) for maximal response