The pFliTrx was amplified using Pfx DNA polymerase (Invitrogen) with the forward primer FP2: 5' GGT CCG TCG AAA ATG ATC GCC CCG ATT CTG GAT 3' and the reverse primer RP2: 5' CGG ACC GCA CCA CTC TGC CCA GAA ATC GAC GAA 3'
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Additionally, new or worsening constipation accompanied by unexplained weight loss (beyond expected therapeutic weight reduction), persistent changes in stool caliber, or alternating constipation and diarrhea warrants evaluation to exclude structural or neoplastic causes, particularly in patients age 45 or older or those with family history of colorectal cancer
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When a medication reduces reward-seeking behavior around food, it naturally raises a clinical question: Could it also influence broader impulsivity patterns
Diarrhea Some individuals experience the opposite, especially during the earliest weeks