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It facilitates the transport of long-chain fatty acids into the mitochondria (the energy-producing parts of cells)
doi: 10.1016/j.bbagrm.2009.11.011 32 GloverD.LittleJ
7 9 Until the mechanisms of apoptosis are elucidated, some natural therapeutics that may decrease programmed cell death, such as melatonin therapy, may be of benefit in PD (see further discussion of melatonin later)
Embodiment 3 [0053] Expression and purification of embodiment 3 fusion protein [0054] Transfer the seed liquid of the recombinant positive engineering strain of Example 2 to LB medium according to the inoculum size of 2%, cultivate it at 37C until OD600=0.6, add 50 l of 0.5mol / L IPTG, induce at 15C for 16 hours, and collect by centrifugation For each 1 g of wet bacteria, resuspend in 10 mL of lysis buffer (50 mM Tris-HCl, 50 mM NaCl, 5% glycerol, pH 8.0, 17 l (10 mg / mL PMSF)), ultrasonicate and centrifuge to obtain supernatant and precipitate
