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Next, they were washed in PTwH (100 PBS 10, 2 ml Tween 20, 1 ml of 10 mg ml 1 heparin stock solution) for 1 10 min, 1 20 min, 1 30 min, 1 1 h, 1 2 h and 1 2 days

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Notably, post-mortem studies revealed reduced activity of respiratory chain complexes in a group of ASD children, suggesting that abnormalities, especially in the frontal, temporal and cerebellar areas (Tang et al., 2013), may cause mitochondrial impairment and contribute to the appearance of the first symptoms (Siddiqui et al., 2016)