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Caplan is amazing, and I personally have benefitted from the exceptional care of Yalda while exploring new treatments

The critical technique during reconstitution involves injecting the bacteriostatic water slowly down the inside wall of the vial rather than directly onto the lyophilized powder

Theres not really much we can hang our hat on, he argues

Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT

Standard dose ranges from 100-300mcg per injection

That is a whopping 14 humans total