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Two failure modes for this protocol are predictable
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Copper accumulation: While copper is essential, excessive accumulation from multiple sources could theoretically lead to oxidative stress and uniform inflammation, distinct from localized purging
The animals were then deeply anesthetized with 3% isoflurane and 50 mg/kg of ketamine followed by a laminectomy at lumbar level L2-L3, corresponding to the sacrocaudal spinal cord
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This suggested that the peptide preferentially targets the expanded vasculature associated with obesity, not normal fat tissue maintenance
