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The sample was extracted by Et 2 O (3) and the aqueous layer was analyzed by RP-HPLC (eluent A 0.1% TFA in water, eluent B 0.1% TFA in CH 3 CN/water 4/1 v/v, C18Xbridge BEH300 5 m (4.6 250 mm) column, gradient 050% B in 15 min, 1 mL min 1 , 30 C, detection at 215 nm)

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Once you are done washing with these buffers, you will immediately rinse with a few column volumes of dIH 2 O followed by a few column volumes with binding buffer, or some kind of buffer with a neutral pH around 7 to 8

NASA's Hubble Space Telescope and other instruments have spotted evidence of sporadic plumes of water vapor that rise perhaps 120 miles (200 kilometers) above Europa's frigid surface

Padowski said