doi:10.3389/fncel.2015.00003 Wright JW, Kawas LH, Harding JW
Introducing cold liquids into a warm vial, or vice-versa, can create thermal shock that compromises the delicate peptide structures
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After dehydration in a series of ascending ethanol concentrations (70%, 80%, 90%, and 100% ethanol), the samples were embedded in paraffin (tissue preparation, T580, FALMA, Tokyo, Japan) using Clear Plus (FALMA)
Little or no induction of GSNOR was apparent, while among the GPX sequences, GPX5 , GPX6 , and GPX7 were most obviously responsive, although some variation was observed between the experiments, including the two cat2 datasets