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After heating in a 10 mM citrate buffer (pH 6.0) at 92C for 15 min, slides were incubated in 3% hydrogen peroxide for 10 min at room temperature

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The fourth flask contains 3% bovine albumin

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This study seeks to investigate the mechanisms involved in APAP-induced hepatotoxicity, as well as the role of 5-HT in the liver's response to APAP toxicity