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Add stop solution 5

The 24-well chamber was fitted with polycarbonate membranes (6.5 mm diameter, 0.33 cm growth area) containing 8.0 m pores

Med Sci Sports Exerc 2004

Additionally, xanthine oxidase is linked to the regulation of nuclear factor of activated T-cells 5 (NFAT5) target genes through ROS, regulating gene expression in different cellular contexts [65]