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Significance was set at p 1 counts/million in at least 4 samples and gene expression distributions were normalized using Bioconductor package edgeR (version 3.40.1) 157

Immunohistochemical (IHC) methods were used to evaluate the expressions of acyl-CoA synthetase long-chain family member 4 (ACSL4) and glutathione peroxidase (GPX4) in the kidney tissues, reverse transcription-quantitative PCR and western blotting were used to assess the levels of ferroptosis marker factors in the kidney tissues and HK-2 cells, and flow cytometry was used to assess cell death

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It tells our brains when we are hungry and when we are full

However, levels of these compounds can vary widely depending on the species of mushroom