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The dark/light cycle were 7am to 7 pm light and 7 pm to 7am dark, ambient temperature was 22 C + /- 2 C and humidity was 4565%

glycyl-L-alpha-glutamyl-L-prolyl-L-prolyl-L-prolylglycyllysyl-L-prolyl-L-alanyl-L-alpha-aspartyl-L-alpha-aspartyl-L-alanylglycyl-L-leucyl-L-valine TB-500: Molecular formula: C212H350N56O78S Molecular weight: 4963.5 Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser GHk-Cu: Molelular formula: C14H22N6O4Cu Molecular weight: 401.91 Sequence: glycyl-l-histidyl-l-lysine

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MCF-7 Cas9-expressing cells were transduced in independent triplicate samples of 1.73 10 8 cells each with the lentiviral whole-genome sgRNA library at a multiplicity of infection of 0.25-0.29, as measured by puromycin resistance, to achieve a representation of 252287 cells per sgRNA