A 35 Ultra Semi diamond knife (Diatome) mounted on a UC7 ultramicrotome (Leica) was used to cut alternating series of sections onto formvar-coated copper mesh grids at two different thicknesses: (i) 60 nm-thick sections were visually inspected to assess ultrastructural preservation and synapse density under an 80kV LEO 912 transmission electron microscope (Zeiss), and (ii) 250 nm-thick sections were coated on both surfaces with Protein A-conjugated 10 nm gold fiducial particles (Cell Microscopy Core Products, University Medical Center Utrecht, The Netherlands) in preparation for transmission electron tomography
Both compounds also decreased the mass of brown adipose tissue in lean and obese mice, suggesting a broader effect on adipose tissue metabolism [2]
Final Thoughts Look, B12 and kidney health isnt something most of us need to lose sleep over
The honest research caveat is that "theoretically additive" is not "experimentally additive" and a researcher who needs to claim synergy must run the combination versus the components in their own model
Whats happening: Tissue strength catches up to function
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