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Cells were then fixed with 4% paraformaldehyde (PFA) for 10 min at room temperature, followed by three washes with 1 PBST (5 min each)

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Activation of PKC can lead to constriction of aorta and BP increasement, via downstream targets of PKC, such as MLCK and CPI-17 (C-kinase potentiated protein phosphatase 1 inhibitor, molecular mass 17 kDa), both of which enhance constriction

The tegument The tegument is a set of proteins that are present in the space between the envelope ant the capsid