To ensure a well-defined Caco-2 cell monolayer prior to initiation of experiments, aliquots of the cell buffers were analyzed by fluorescence to determine the transport of the impermeable dye Lucifer yellow
Reconstitution of a single vial rather than two reduces preparation steps, minimises the risk of dosing errors, and simplifies inventory management considerations that become meaningful in high-throughput preclinical settings
No commercial relationship, sponsorship, approval, or association with any trademark owner should be inferred
Another well-documented dietary supplement in this field is melatonin (MLT), which is mentioned extensively in the context of dry AMD due to its inhibitory effect on ferroptosis in both RPE cells and PR cells
Toxicology 445 , 152599 (2020)
Grabowski ZR, Rotkiewicz K, Rettig W (2003) Structural changes accompanying intramolecular electron transfer: focus on twisted intramolecular charge-transfer states and structures