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Multiple strategies were employed to minimize observed and possible threats to the reliability and validity of the survey

Disulfide bonds were then reduced with tris(2-carboxyethyl)phosphine hydrochloride and proteins precipitated with 10% sulfosalicylic acid

The treatment is usually given over multiple sessions, each lasting 2030 minutes

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What the research shows: Frye et al., 2016: 48 children with autism + language impairment received leucovorin (0.52 mg/kg/day, up to 50 mg/day) for 12 weeks

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