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intranasal doses of approximately 120 mcg/kg used in animal research Timing: Evening administration, typically 30 to 60 minutes before intended sleep Onset: Sleep pressure reported within approximately 130 minutes of administration in human studies Duration: Effects on sleep architecture observed with both single doses and multi-day protocols Regarding cycling, the evidence is mixed

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Therefore DIO mice were treated daily with escalating doses of P5 or equimolar doses of Ex4 for 4 weeks

S., & Terrell, J