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Proteins were then transferred onto nitrocellulose membranes (Amersham Protran 0.45 m) using a Trans-Blot SD semi-dry electrophoretic transfer cell (Bio-Rad) using 1 NuPage transfer buffer (Invitrogen, NP00061) with 10% methanol

Singla-Pareek SL, Kaur C, Kumar B, Pareek A, Sopory SK (2020) Reassessing plant glyoxalases: large family and expanding functions

NOTE: Johns Hopkins University Press, publisher of The Lupus Encyclopedia, is a nonprofit publisher

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"At this point, I don't fit into any my clothes," Mooney, now 54, told "Good Morning America," adding that she felt like her body was "turning against" her