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Gentle swirling (not vortexing) is recommended to preserve peptide structure

At a lower pH, peptides are usually protonated and strongly bind with the column

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In the 58.3% of patients with vomiting at baseline, all three endpoints also improved and, in addition, there was reduction in the composite score of nausea, abdominal pain, bloating and early satiety [123,124,125,126]