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Apart from lipid biosynthesis, another critical pro-inflammatory process that NADPH supports in myeloid cells is the production of cytotoxic, diffusive reactive radicals, such as reactive oxygen species (ROS) via NADPH oxidases (NOXes), as well as nitric oxide ( NO) via nitric oxide synthases (NOSes)

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After washing with 70% ethanol, the RNA was solubilized in diethyl pyrocarbonate (DEPC)-treated RNase-free double-distilled water and quantified by measuring the absorbance in a UV-2401PC spectrophotometer (Shimadzu) at 260 nm