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We used 15 N-labeled FOXO4 FHD and p53 TAD with S-(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl methanesulfonothioate (MTSL)-labeled Cys46 for the PRE experiments

While a constant exposure of quinone is not necessarily representative of an in vivo scenario, it is however, much more representative of an in vitro situation, whereby a constant source of quinone is essentially available in the extracellular media during cell culture 27

Glutathione itself is a powerful antioxidant that is already formed and used throughout the body to help protect cells from oxidative stress

In addition to carcinogenesis, toxicity is associated with mycotoxins

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Many different affinity tags have been developed (Terpe, 2002)