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An in vitro study (Teixeira and Rumjanek, 2014) showed that human monocytes treated with ouabain at a concentration of 100 nM produced heightened levels of early markers of monocyte activation: IL-1, TNF-, IL-10, vascular endothelial growth factor (VEGF), surface monocyte activation markers such as CD69, a transmembrane type II C-lectin receptor, and HLA-DR (Human Leukocyte AntigenDR isotype) (Ziegler et al., 1994)

In a longer study, the effect would likely be even larger

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