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Although egg utilization is impacted by loss at thawing and lower rates of fertilization when compared to fresh eggs, embryos created from fresh and frozen eggs have similar pregnancy rates

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TP53 coding DNA sequence (CDS) purification from gDNA TP53 CDS was amplified as previously described [19], with the exception of the last purification step with AMPure XP (Beckman Coulter, Indianapolis, IN, USA)

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[20] Over the course of a 52 week double blind study to detect the efficacy and safety of dulaglutide monotherapy versus metformin in type 2 diabetes, the most common side effects seen were nausea, diarrhea, and vomiting [20]