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in the evening, before bed nasal application 1-2 sprays per nostril Scientific sources Delta-sleep-inducing peptide: a review scientific review A review article summarizing studies on DSIP, describing its impact on promoting delta sleep (deep sleep) in various species (rabbits, mice, rats, humans)
But as you age, you keep doing that with the same pool of mitochondria, then you're going to get a bunch of old mitochondria mixing old stuff together
Experimental research suggests DSIP may influence central neurotransmitter balance, potentially supporting inhibitory tone without direct receptor agonism