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We have reported that serum total IGF-I decreased 1 week and 3 months after bariatric surgery, whereas serum bioactive IGF-I remained unchanged during that period, and after 12 months, both variables returned to pre-surgery levels (117)

doi: 10.1021/jf5024615 19

Cells were lysed using repeated freeze/thaw cycles a minimum of 5 times in 1 Gradient Buffer (0.1 M Tris, 0.5 M NaCl, 0.1 M MgCl 2 )

Results were generally consistent with those from our companion survey of GLP-1 consumers and employers

Over time, these side effects decreased or became milder as participants adjusted to a stable dose