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Long Y, Zeng F, Shi J, Tian H, Chen T

Lima WG, Martins-Santos ME, Chaves VE

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Slides containing retinal sections were deparaffinized by immersing the slides in various subsequent ethanol dilutions(100%, 95%,70% and 50%), then rinsed with phosphate-buffered saline, pH 7.4 (PBS, Quality Biological, 119-069-101), followed by incubation in blocking buffer containing 0.5% normal donkey serum (Abcam, ab7475), 0.5% bovine serum albumin (BSA, GoldBio, A-420-100), 0.1% Triton X-100 (Sigma, T8787-100) in PBS pH 7.4 for 2 h at room temperature 28

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It occurs due to various factors such as the dosage of the medication, body mass index, metabolism, timing of meals, food choices, and hydration