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Interscapular brown adipose tissue was homogenized in Tris-ethylenediaminetetraacetic acid (EDTA) buffer (10 mM Tris and 1 mM EDTA, pH 7.4) with 1% protease inhibitor (Nacalai Tesque, Kyoto, Japan) on ice

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Simultaneously, glucagon release is suppressed

Glutathione has long been recognized as a central intracellular antioxidant, and ongoing clinical conversations continue to broaden how its role is understood across oxidative stress, inflammation, neurotransmission, and cellular resilience