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doi: 10.2147/LCTT.S90694 63 WiebeJPLewisMJ

The isolated PHH preparations (either from fresh tissue from Dresden or Leipzig Hospital or commercially available frozen hepatocytes) were enriched for both EpCAM-negative (hepatocytes) and EpCAM-positive (cholangiocytes) by MACS using an anti-human CD326 antibody (BioLegend) and anti-biotin microbeads (Ultra Pure, Miltenyi) following the manufacturers instructions

Patients should avoid injecting into the same spot repeatedly and maintain a rotation schedule, waiting at least one week before returning to a previously used site

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