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For transfection, the cells were cultured until 70% confluency and transfected with the pCMV-2B FLAG-GST expression vector using Lipofectamine 3000 reagent in Opti-MEM (Gibco) medium according to the manufacturers protocol (Invitrogen)

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Oxytocin Peptide and Autism The main aim of this clinical study (9) was to evaluate what action, if any, the peptide may exert on research models of autism